维生素C对肉鸡缺氧肺动脉平滑肌细胞缺氧诱导因子-1α转录水平的影响

doi:10.11843/j.issn.0366-6964.2016.02.024

Abstract

Abstract:

This study was conducted to investigate the effects of Vitamin C (VC) on hypoxia inducible factor-1α (HIF-1α)，vascular endothelial growth factor (VEGF) and its receptor 2 (VEGFR2) mRNA transcription of broiler pulmonary artery smooth muscle cells（PASMCs）under hypoxia induced by CoCl₂.PASMCs from healthy AA broilers at 21 days of age were isolated，cultured and identified.A cell model of hypoxia was established by using CoCl₂.On this basis，PASMCs were treated by 7 different concentrations of VC (0，50，100，200，500，1 000 and 1 500 μmol•L^-1) and 5 processing time (6，12，24，48 and 72 h)，total 35 treatments with six replicates.The results indicated that the PASMCs of broilers were successfully separated and cultured in vitro.As for hypoxia model of PASMCs，the relative transcription of HIF-1α，VEGF，VEGFR2 and cell proliferation capacity significantly increased when PASMCs were after 48 h treatment by CoCl₂with 250 μmol•L^-1 (P<0.05).The relative transcription of HIF-1α，VEGFR2 in hypoxia PASMCs were significantly decreased by VC with 500 or 1 000 μmol•L^-1 after 48 or 72 h (P<0.05).Whereas 1 500 μmol•L^-1 VC significantly increased the relative transcription of HIF-1α，VEGF，VEGFR2 in hypoxia PASMCs (P<0.05).These results suggested that anoxia could up-regulate the relative expression of hypoxia related genes and induce abnormally proliferation of PASMCs.While VC could reduce the sensitivity to oxygen signals of hypoxia PASMCs，and further down-regulate the relative expression of hypoxia related genes.

CLC Number:

S852.2

CAO Lin，ZENG Qiu-feng，ZHANG Ke-ying，DING Xue-mei，BAI Shi-ping，LUO Yu-heng，WANG Jian-ping，XUAN Yue，SU Zhuo-wei. Effects of Vitamin C on the Transcription of Hypoxia Inducible Factor-1α of Broiler Pulmonary Artery Smooth Muscle Cells under Hypoxia[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(2): 388-396.

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Effects of Vitamin C on the Transcription of Hypoxia Inducible Factor-1α of Broiler Pulmonary Artery Smooth Muscle Cells under Hypoxia

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